Journal of Agriculture & Life Sciences

Effects of Various Concentrations of Dietary Lead on Antioxidant Gene Expressions in Different Tissues of Pigs
Kondreddy Eswar Reddy, Sung Dae Lee, Dong Wook Kim, Young Kyun Oh, Min Ji Kim, Youl Chang Baek, JinYoung Jeong, Min Seok Kim, Dong Woon Kim, Hyun-Jeong Lee*, Yun Jeong Do

Abstract
Twenty weaned pigs were allocated to four dietary Pb levels for four weeks, such as control, low-level (LL, 10 mg kg-1), maximum tolerable level (MTL, 25mg kg-1) and high toxic level (HTL, 250 mg kg-1). The differential expression of twelve antioxidant genes encoding stressor biomarkers was investigated by quantitative reverse transcription-PCR in muscle, liver and kidney of pigs. The expression results indicated that, the antioxidant enzymes such as GPx1, CAT, GR, SOD, G6PD, TXNRD1 and PGC-1α were gradually down regulated (P<0.05) in muscle, liver and kidney of LL, MTL and HTL dietary Pb treatment groups. CYP1A1 and MT1A gene expression rates were higher in LL and MTL dietary treatments and lower in HTL, when compared with the control group. The TP53 regulates reactive oxygen species generation, was affected with increasing (P<0.05) dietary Pb concentrations. The Pb accumulation rate in tissues are in the order of Kidney> Liver > muscle. As a result, dysfunction occurred in those tissues after dietary Pb supplementation according to the accumulation rate and there was an inhibition of the antioxidant system.

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