Journal of Agriculture & Life Sciences

Antioxidant Activity and Persistence of Cottonseed Protein and Oil from Two Cultivars as Determined by Their Ability to Scavenge Peroxyl and Alkoxyl Radicals
Dipaloke Mukherjee, Zahur Z. Haque

Abstract
Short-term antioxidant activity (AA) and long-term antioxidative persistence (AP) of protein and oil fractions extracted from two cultivars of cotton, namely PIMA 3.79 056-7900 (Gossypium barbadense) and TM1 123-1452 (G. hirsutum) were investigated. This was based on their efficacy to scavenge peroxyl and alkoxyl radicals generated in-vitro by pyrolysis of 2,2’-Azobis(2-methylpropionamidine) dihydrochloride. Luminal induced chemiluminescence resulting from unquenched radicals was used to monitor the relative changes in such efficiency in real time. Results showed significantly greater (P<0.05) AA and AP of protein and oil extracts from both the cultivars compared to the control. Unlike the proteins, the oil extracts did not exhibit a significant difference among themselves regarding AA.They also showed a considerable deterioration in their AP. Such decline in the antioxidative efficacy of the hydrophobic non-protenaceous ether extractfrom cottonseeds has not been reported before.

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