Journal of Agriculture & Life Sciences

Effect of Sugars on mRNA Expression of 11β-HSD1in the Hypothalamus of Rats after 24 hour Exposure
Changhui Zhao, Anna E. Tschiffely, Thomas W. Castonguay

Abstract
Fructose can quickly increase the 11β-HSD1 expression in both the liver and adipose tissue of rats. The intra cellular glucocorticoids regeneratedby11β-HSD1 increase the risk of obesity development. However, how sugars affect the expression of hypothalamic 11β-HSD1 is not clear. In the current paper, we treated Sprague Dawley rats with 15% of different sugar solutions including glucose, sucrose, high fructose corn syrup (HFCS) and fructose. After 24 hour exposure, we sampled portions of the par ventricular hypothalamic nucleus (PVN), ventromedial hypothalamus (VMH) and lateral hypothalamus (LH). We then measured mRNA expression of 11β-HSD1 in these three regions of hypothalamus using quantitative PCR techniques. Results showed that although sugars have no significant effect on 11β-HSD1 expression in the PVN, VMH or LH compared to control, HFCS did increase 11β- HSD1 expression compared with glucose and sucrose group in both the PVN and VMH.

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